Prevalence of Multidrug-Resistant Listeria monocytogenes in Dairy Products with Reduction Trials Using Rosmarinic Acid, Ascorbic Acid, Clove, and Thyme Essential Oils

Author:

Elafify Mahmoud1,Elabbasy Mohamed Tharwat23ORCID,Mohamed Rasha Samir4,Mohamed Esraa A.5,Saad Eldin Walaa Fathy6,Darwish Wageh Sobhy3ORCID,Eldrehmy Essam Hassan78,Shata Radwa Reda1

Affiliation:

1. Department of Food Hygiene and Control, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt

2. Public Health Department, College of Public Health and Health Informatics, Ha’il University, Ha’il, Saudi Arabia

3. Food Control Department, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, Egypt

4. Department of Agricultural Microbiology, Faculty of Agriculture, Cairo University, Cairo 12613, Egypt

5. Department of Medical Microbiology and Immunology, Faculty of Medicine, Tanta University, Tanta, Egypt

6. Educational Veterinary Hospital, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, Egypt

7. Department of Biology, Turabah University College, Taif University, Taif, Saudi Arabia

8. Department of Microbiology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt

Abstract

Continuous monitoring of Listeria spp., particularly Listeria monocytogenes, in foods is a mandatory task for food safety and microbiology sectors. This study aimed to determine the prevalence and antimicrobial resistance patterns of L. monocytogenes in milk and dairy products retailed in Egypt. Furthermore, an experimental trial was conducted to investigate the antilisterial effects of some phytochemicals. A total of 200 samples (market raw milk, Kareish cheese, Damietta cheese, and plain yoghurt, 50 each) were collected and examined for detection of Listeria spp. The results revealed that 8, 12, 1, and 0 samples of market raw milk, Damietta cheese, Kareish cheese, and plain yoghurt were contaminated with Listeria spp., respectively. Antimicrobial sensitivity testing revealed that all L. monocytogenes isolates (15) were resistant to streptomycin and erythromycin. Molecular analysis revealed that 86.67% of L. monocytogenes harbored hylA virulent gene. Use of rosmarinic acid, ascorbic acid, thyme, and clove essential oils significantly ( P < 0.05 ) reduced L. monocytogenes growth in soft cheese—artificially contaminated with L. monocytogenes throughout a 4-week incubation period. In conclusion, strict hygienic conditions should be adopted during the preparation and distribution of dairy products. In addition, rosmarinic acid, ascorbic acid, clove, and thyme essential oils are good candidates as food preservatives with antilisterial activities.

Funder

University of Hail

Publisher

Hindawi Limited

Subject

Safety, Risk, Reliability and Quality,Food Science

Reference56 articles.

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3