Effects ofStreptococcus sanguinisBacteriocin on Cell Surface Hydrophobicity, Membrane Permeability, and Ultrastructure ofCandidaThallus

Author:

Ma Shengli1,Zhao Yingnan2,Xia Xue2,Dong Xue2,Ge Wenyu2,Li Hui3

Affiliation:

1. Department of Stomatology, Hospital of Heilongjiang Province, Harbin 150036, China

2. University of Jiamusi, Jiamusi 154002, China

3. The 2nd Affiliated Hospital of Harbin Medical University, Harbin 150001, China

Abstract

Candida albicans(C.a) andCandida tropicalis(C.t) were treated withStreptococcus sanguinisbacteriocin (S.s bacteriocin), respectively; the bacteriostatic dynamics of S.s bacteriocin, their effects on cell surface hydrophobicity, leakage of inorganic phosphorus and macromolecular substance, cytosolic calcium concentration, and ultrastructure changes ofCandidathallus were detected and analyzed. The results showed that inhibitory effect of S.s bacteriocin on C.a and C.t reached peak level at 24 h, the cell-surface hydrophobicity decreased significantly (P< 0.05) after S.s bacteriocin treatment, and there was leakage of cytoplasmic inorganic phosphorus and macromolecular substance from C.a and C.t; cytosolic calcium concentration decreased greatly. After 24 h treatment by S.s bacteriocin, depressive deformity and defect could be found in the cell surface of C.a and C.t; the thallus displayed irregular forms: C.a was shrunken, there was unclear margins abutting upon cell wall and cell membrane, nucleus disappeared, and cytoplasm was inhomogeneous; likewise, C.t was first plasmolysis, and then the cytoplasm was shrunk, the ultrastructure of cell wall and cell membrane was continuously damaged, and the nucleus was karyolysis. It was illustrated that S.s bacteriocin had similar antifungal effect on C.a and C.t; their cell surface hydrophobicity, membrane permeability, and ultrastructure were changed significantly on exposure to S.s bacteriocin.

Funder

Natural Science Foundation of the Government of Heilongjiang Province

Publisher

Hindawi Limited

Subject

General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

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