Production of Bioactive Compounds by Actinomycetes and Their Antioxidant Properties

Author:

Janardhan Avilala1,Kumar Arthala Praveen1,Viswanath Buddolla1,Saigopal D. V. R.1,Narasimha Golla1

Affiliation:

1. Department of Virology, Sri Venkateswara University, Tirupati, Andhra Pradesh 517502, India

Abstract

An actinomycete was isolated from mangrove soil collected from Nellore region of Andhra Pradesh, India, and screened for its ability to produce bioactive compounds. The cultural, morphological, and biochemical characters and 16S rRNA sequencing suggest that the isolated strain is Nocardiopsis alba. The bioactive compounds produced by this strain were purified by column chromatography. The in vitro antioxidant capacity of the isolated compounds (fractions) was estimated and fraction F2 showed very near values to the standard ascorbic acid. The potential fraction obtained by column chromatography was subjected to HPLC for further purification, then this purified fraction F2 was examined by FTIR, NMR, and mass spectroscopy to elucidate its chemical structure. By spectral data, the structure of the isolated compound was predicted as “(Z)-1-((1-hydroxypenta-2,4-dien-1-yl)oxy)anthracene-9,10-dione.”

Publisher

Hindawi Limited

Subject

Automotive Engineering

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