Radical-Scavenging Activity and Ferric Reducing Ability ofJuniperus thurifera(L.),J. oxycedrus(L.),J. phoenicea(L.) andTetraclinis articulata(L.)

Abstract

Objective. The aim of this work is to study and compare the antioxidant properties and phenolic contents of aqueous leaf extracts ofJuniperus thurifera,Juniperus oxycedrus,Juniperus Phoenicea,andTetraclinis articulatafrom Morocco.Methods. Antioxidant activities of the extracts were evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical-scavenging ability, Trolox equivalent antioxidant capacity (TEAC), and ferric reducing antioxidant power (FRAP) assays. Also the total phenolic and flavonoids contents of the extracts were determined spectrophotometrically.Results. All the extracts showed interesting antioxidant activities compared to the standard antioxidants (butylated hydroxytoluene (BHT), quercetin, and Trolox). The aqueous extract ofJuniperus oxycedrusshowed the highest antioxidant activity as measured by DPPH, TEAC, and FRAP assays with IC50values of17.91±0.37 μg/mL,19.80±0.55 μg/mL, and24.23±0.07 μg/mL, respectively. The strong correlation observed between antioxidant capacities and their total phenolic contents indicated that phenolic compounds were a major contributor to antioxidant properties of these plants extracts.Conclusion. These results suggest that the aqueous extracts ofJuniperus thurifera,Juniperus oxycedrus,Juniperus phoenicea,andTetraclinis articulatacan constitute a promising new source of natural compounds with antioxidants ability.