Anti-inflammatory Effects of S. cumini Seed Extract on Gelatinase-B (MMP-9) Regulation against Hyperglycemic Cardiomyocyte Stress

Author:

Atale Neha1ORCID,Mishra Chandra Bhushan2ORCID,Kohli Shrey3,Mongre Raj Kumar2ORCID,Prakash Amresh4ORCID,Kumari Sweta5,Yadav Umesh C. S.6ORCID,Jeon Raok2ORCID,Rani Vibha1ORCID

Affiliation:

1. Department of Biotechnology, Jaypee Institute of Information Technology, A-10, Sector-62, Noida, 201307 Uttar Pradesh, India

2. College of Pharmacy, Sookmyung Women’s University, Hyochangwon-gil 52, Yongsan-13 Gu, Seoul 140-742, Republic of Korea

3. Institute for Laboratory Medicine, Clinical Chemistry and Molecular Diagnostics, University of Leipzig (Medical Faculty), Leibigstr. 27, 04103 Leipzig, Germany

4. Amity Institute of Integrative Sciences and Health, Amity University, Gurgaon 122413, Haryana, India

5. Division of Biochemistry, Indian Agricultural Research Institute (IARI), Pusa Campus, 110012, New Delhi, India

6. School of Life Sciences, Central University of Gujarat, Sector-30, Gandhinagar, 382030 Gujarat, India

Abstract

Black berry (Syzygium cumini) fruit is useful in curing diabetic complications; however, its role in diabetes-induced cardiomyopathy is not yet known. In this study, we investigated the regulation of gelatinase-B (MMP-9) by S. cumini methanol seed extract (MSE) in diabetic cardiomyopathy using real-time PCR, RT-PCR, immunocytochemistry, gel diffusion assay, and substrate zymography. The regulatory effects of MSE on NF-κB, TNF-α, and IL-6 were also examined. Identification and estimation of polyphenol constituents present in S. cumini extract were carried out using reverse-phase HPLC. Further, in silico docking studies of identified polyphenols with gelatinase-B were performed to elucidate molecular level interaction in the active site of gelatinase-B. Docking studies showed strong interaction of S. cumini polyphenols with gelatinase-B. Our findings indicate that MSE significantly suppresses gelatinase-B expression and activity in high-glucose- (HG-) stimulated cardiomyopathy. Further, HG-induced activation of NF-κB, TNF-α, and IL-6 was also remarkably reduced by MSE. Our results suggest that S. cumini MSE may be useful as an effective functional food and dietary supplement to regulate HG-induced cardiac stress through gelatinase.

Funder

Ministry of Science, ICT and Future Planning

Publisher

Hindawi Limited

Subject

Cell Biology,Aging,General Medicine,Biochemistry

Reference69 articles.

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