Differential Expression of Serum Proteins in Rats with Allergic Asthma: A Study Based on the Nanoliter Two-Dimensional Liquid Chromatography Technique

Author:

Yan Xingke1,Zhang Ao1ORCID,Yu Lu2,Chen Cheng3,Cui Haifu4

Affiliation:

1. College of Acupuncture, Moxibustion and Tuina, Gansu University of Chinese Medicine, Lanzhou 730000, China

2. Jilin Provincial Jilin Hospital of Integrated Traditional Chinese and Western Medicine, Jilin 132021, China

3. Daqing Chinese Medicine Hospital, Daqing 163311, China

4. Hunchun Chinese Medicine Hospital, Hunchun 133300, China

Abstract

Objective. To investigate the pathogenesis of allergic asthma via the nanoliter two-dimensional liquid chromatography (nano-2D-LC) technique.Method. 24 Wistar rats were randomized into 3 groups: the blank-control group (A), the restrained group (B), and the asthma model group (C). The nanoliter two-dimensional liquid chromatography (nano-2D-LC) technique was used to study the differential protein expressions of the serum in asthmatic rats.Results. Compared with the restrained group, the data from the asthma model group displayed a distinctive peak, that is, peak number 13 (94.731 min). The data also displayed three missing peaks in the asthma group, that is, peak number 1 (77.489 min), peak number 2 (78.418 min), and peak number 5 (80.533 min), suggesting that the corresponding peptides might be related to the pathogenesis of asthma. Compared with the blank-control group, the restrained group showed 4 new peaks, that is, peak number 2 (78.418 min), peak number 4 (79.398 min), peak number 5 (80.533 min), and peak number 7 (81.824 min). The restrained group also displayed a missing peak, that is, peak number 3 (78.804 min), indicating that those 5 polypeptides might be related to the binding-induced stress stimuli.Conclusion. The study suggests that the pathogenesis of allergic asthma is closely related to abnormal levels of proteins; however, future animal experiments should identify the specific protein expressions caused by stress factors.

Funder

State Administration of Traditional Chinese Medicine

Publisher

Hindawi Limited

Subject

Pulmonary and Respiratory Medicine

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