The Role of Overexpressed Apolipoprotein AV in Insulin-Resistant Hepatocytes

Abstract

In this paper, we sought to explore the relationship between apolipoprotein AV (APOAV) overexpression and insulin resistance in hepatocytes. The insulin-resistant HepG2 cell model was constructed, and then,APOAV-overexpressed HepG2 cells (B-M) were induced by infecting with a recombinant adenovirus vector. Microarray data were developed from B-M samples compared with negative controls (A-con), and the microarray data were analyzed by bioinformatic methods.APOAV-overexpression induced 313 upregulated genes and 563 downregulated ones in B-M sample. The differentially expressed genes (DEGs) were significantly classified in fat digestion and absorption pathway. Protein-protein interaction network was constructed, andAGTR1(angiotensin II receptor type 1) andP2RY2(purinergic receptor P2Y, G-protein coupled 2) were found to be the significant nodes closely related with G-protein related signaling. Additionally, overexpression ofAPOAVcould change the expression of Glut4 and release the insulin resistance of hepatic cells. Thus,APOAVoverexpression may prevent the insulin resistance in liver cells by mediating the genes such asAGTR1andP2RY2.