Pleiotropic Effects of Biguanides on Mitochondrial Reactive Oxygen Species Production

Author:

Pecinova Alena1,Drahota Zdenek1,Kovalcikova Jana1,Kovarova Nikola1,Pecina Petr1,Alan Lukas1,Zima Michal1,Houstek Josef1,Mracek Tomas1ORCID

Affiliation:

1. Institute of Physiology of the Czech Academy of Sciences, Vídeňská, 1083 Prague, Czech Republic

Abstract

Metformin is widely prescribed as a first-choice antihyperglycemic drug for treatment of type 2 diabetes mellitus, and recent epidemiological studies showed its utility also in cancer therapy. Although it is in use since the 1970s, its molecular target, either for antihyperglycemic or antineoplastic action, remains elusive. However, the body of the research on metformin effect oscillates around mitochondrial metabolism, including the function of oxidative phosphorylation (OXPHOS) apparatus. In this study, we focused on direct inhibitory mechanism of biguanides (metformin and phenformin) on OXPHOS complexes and its functional impact, using the model of isolated brown adipose tissue mitochondria. We demonstrate that biguanides nonspecifically target the activities of all respiratory chain dehydrogenases (mitochondrial NADH, succinate, and glycerophosphate dehydrogenases), but only at very high concentrations (10−2–10−1 M) that highly exceed cellular concentrations observed during the treatment. In addition, these concentrations of biguanides also trigger burst of reactive oxygen species production which, in combination with pleiotropic OXPHOS inhibition, can be toxic for the organism. We conclude that the beneficial effect of biguanides should probably be associated with subtler mechanism, different from the generalized inhibition of the respiratory chain.

Funder

Ministerstvo Školství, Mládeže a Tělovýchovy

Publisher

Hindawi Limited

Subject

Cell Biology,Ageing,General Medicine,Biochemistry

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