The Potential Health Benefits of Polyphenol-Rich Extracts fromCichorium intybusL. Studied on Caco-2 Cells Model

Author:

Azzini Elena1,Maiani Giuseppe1,Garaguso Ivana1,Polito Angela1,Foddai Maria S.1,Venneria Eugenia1,Durazzo Alessandra1,Intorre Federica1,Palomba Lara1,Rauseo Maria L.1,Lombardi-Boccia Ginevra1,Nobili Fabio1

Affiliation:

1. Council for Agricultural Research and Economics (CREA), Research Center for Food and Nutrition, Via Ardeatina 546, 00178 Rome, Italy

Abstract

Phytochemicals can exert their bioactivity without reaching the systemic circulation; scarcely absorbed antioxidants might reach the large bowel contributing to protection from oxidative damage-induced gastrointestinal diseases. In the present work, we aimed to study the relationship between potential activity of polyphenol-rich extracts fromCichorium intybusL. and changes in morphological characteristics on Caco-2 cells. Phytochemicals content (carotenoids and flavonoids) and total antioxidant activity of Red Chicory of Treviso and Variegated Chicory of Castelfranco were evaluated. The bioactivity of polyphenol-rich extracts from chicories was studied inin vitroCaco-2 cell monolayers model. Morphological characteristics changes to test the antioxidant and/or prooxidant effect were verified by histological analysis and observed by Electronic Scansion Microscopy (SEM). On Caco-2 cell model, the polyphenols fractions from chicories have indicated a moderate antioxidant behavior until 17 μM concentration, while 70 μM and 34 μM exert cytotoxic effects for Treviso’s and Castelfranco’s Chicory, respectively, highlighted by TEER decreasing, increased permeability, and alteration of epithelium. Our findings support the beneficial effects of these products in counteracting the oxidative stress and cellular damage, inducedin vitroon Caco-2 cell model, through interaction with the mucopolysaccharide complexes in the glycocalyx, maintainingin vivoa healthy and effective intestinal barrier.

Funder

Italian Ministry of Agriculture, Food, and Forestry Policies

Publisher

Hindawi Limited

Subject

Cell Biology,Ageing,General Medicine,Biochemistry

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