Metagenomic Analysis of Gut Microbiome in Gout Patients with Different Chinese Traditional Medicine Treatments

Author:

Xie Jingjing12,Wang Jing3,Zhao Fang4,Qiu Xia2,Chen Jianwei45,Jia Yangyang4,Qin Panpan5,Zhu Yuanfang4ORCID,Zhang Jianyong12ORCID,Wang Binbin6ORCID

Affiliation:

1. The Fourth Clinical Medical College of Guangzhou University of Chinese Medicine, Shenzhen, Guangdong, China

2. Department of Rheumatism, Shenzhen Traditional Chinese Medicine Hospital, Shenzhen, Guangdong, China

3. Department of Medical Genetics and Developmental Biology, School of Basic Medical Sciences, Capital Medical University, Beijing 10069, China

4. BGI-Qingdao, BGI-Shenzhen, Qingdao 266555, China

5. Qingdao-Europe Advanced Institute for Life Sciences, BGI-Shenzhen, Qingdao 266555, China

6. Center for Genetics, National Research Institute for Family Planning, Beijing, China

Abstract

Introduction. Changes in eating habits have made gout a metabolic disease of increasing concern. Previous studies have indicated that there are significant differences in species composition and abundance of gut microbiome in gout patients compared with average. Considering that traditional Chinese medicine has a momentous effect in treating gout, the research study aimed to explore the differences of genomic and metabolomics of gut microbiome before and after traditional Chinese medicine treatment in patients with gout. Method. 30 patients with gout and 29 matched controls were recruited of which 16 patients took H treatment and 14 patients took T treatment. Stools were collected twice for patients before and after treatment and only once for controls. A total of 89 samples were annotated with metagenomic species and functions, and the enrichment analysis of differential genes and KO pathway was carried out. Result. The results showed a decrease in the diversity of gut microbiome in gout patients and the gene abundance and metabolomics had great differences among study groups. The number of bacterial genera also had significant differences among treatment groups. Moreover, among different groups, the regulation of different species was variously correlated. The correlation between species and clinical laboratory indicators in the rising group was stronger than that in the decreasing group and the upregulation of some strain was related to the content of urea nitrogen. Conclusion. After the traditional Chinese medicine treatment, the glutathione pathway was significantly enriched and some pathogenic bacteria were significantly inhibited. The study suggests that traditional Chinese medicine treatment may exert its therapeutic effect by inhibiting relevant pathways.

Funder

Science and Technology Planning Project of Shenzhen Municipality

Publisher

Hindawi Limited

Subject

Complementary and alternative medicine

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