Affiliation:
1. College of Life Sciences and Health Wuhan University of Science and Technology Wuhan 430065 China
2. State Key Laboratory of Virology Wuhan Institute of Virology Center for Biosafety Mega‐Science Chinese Academy of Sciences Wuhan 430071 China
3. Faculty of Synthetic Biology Shenzhen Institute of Advanced Technology Chinese Academy of Sciences Shenzhen 518055 China
4. National Laboratory of Biomacromolecules Institute of Biophysics Chinese Academy of Sciences Beijing 100101 China
5. University of Chinese Academy of Sciences Beijing 100049 China
Abstract
BackgroundAs one of the representative protein materials, protein nanocages (PNCs) are self‐assembled supramolecular structures with multiple advantages, such as good monodispersity, biocompatibility, structural addressability, and facile production. Precise quantitative functionalization is essential to the construction of PNCs with designed purposes.ResultsWith three modifiable interfaces, the interior surface, outer surface, and interfaces between building blocks, PNCs can serve as an ideal platform for precise multi‐functionalization studies and applications. This review summarizes the currently available methods for precise quantitative functionalization of PNCs and highlights the significance of precise quantitative control in fabricating PNC‐based materials or devices. These methods can be categorized into three groups, genetic, chemical, and combined modification.ConclusionThis review would be constructive for those who work with biosynthetic PNCs in diverse fields.
Funder
National Natural Science Foundation of China
Chinese Academy of Sciences
Subject
Applied Mathematics,Computer Science Applications,Biochemistry, Genetics and Molecular Biology (miscellaneous),Modeling and Simulation