Designed proteins assemble antibodies into modular nanocages-Reference-Cited by-同舟云学术

Designed proteins assemble antibodies into modular nanocages

Published:2021-04-02 Issue:6537 Volume:372 Page:
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Divine Robby¹²^ORCID,Dang Ha V.¹^ORCID,Ueda George¹²^ORCID,Fallas Jorge A.¹²^ORCID,Vulovic Ivan¹²^ORCID,Sheffler William²^ORCID,Saini Shally¹³^ORCID,Zhao Yan Ting¹³⁴,Raj Infencia Xavier¹³^ORCID,Morawski Peter A.⁵^ORCID,Jennewein Madeleine F.⁶^ORCID,Homad Leah J.⁶^ORCID,Wan Yu-Hsin⁶^ORCID,Tooley Marti R.²,Seeger Franziska¹²,Etemadi Ali²⁷,Fahning Mitchell L.⁵^ORCID,Lazarovits James¹²^ORCID,Roederer Alex²^ORCID,Walls Alexandra C.¹^ORCID,Stewart Lance²^ORCID,Mazloomi Mohammadali⁷^ORCID,King Neil P.¹²^ORCID,Campbell Daniel J.⁵^ORCID,McGuire Andrew T.⁶⁸^ORCID,Stamatatos Leonidas⁶⁸^ORCID,Ruohola-Baker Hannele¹³^ORCID,Mathieu Julie³⁹^ORCID,Veesler David¹^ORCID,Baker David¹²¹⁰^ORCID

Affiliation:

1. Department of Biochemistry, University of Washington, Seattle, WA 98195, USA.

2. Institute for Protein Design, University of Washington, Seattle, WA 98195, USA.

3. Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle, WA 98109, USA.

4. Oral Health Sciences, School of Dentistry, University of Washington, Seattle, WA 98195, USA.

5. Benaroya Research Institute, Seattle, WA 98101, USA.

6. Vaccines and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98019, USA.

7. Medical Biotechnology Department, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences (TUMS), Tehran, Iran.

8. Department of Global Health, University of Washington, Seattle, WA 98195, USA.

9. Department of Comparative Medicine, University of Washington, Seattle, WA 98195, USA.

10. Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195, USA.

Abstract

Integrating form and function for design Antibodies are broadly used in therapies and as research tools because they can be generated against a wide range of targets. Efficacy can often be increased by clustering antibodies in multivalent assemblies. Divine et al. designed antibody nanocages from two components: One is an antibody-binding homo-oligomic protein and the other is the antibody itself. Computationally designed proteins drive the assembly of antibody nanocages in a range of architectures, allowing control of the symmetry and the antibody valency. The multivalent display enhances antibody-dependent signaling, and nanocages displaying antibodies against the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein effectively neutralize pseudovirus. Science , this issue p. eabd9994

Funder

National Science Foundation

National Institutes of Health

National Center for Advancing Translational Sciences

Wellcome

Howard Hughes Medical Institute

Washington Research Foundation

National Institute of Allergy and Infectious Diseases

National Institute of General Medical Sciences

U.S. Department of Defense

American Heart Association

Wu Tsai Translational Investigator Fund