Affiliation:
1. College of Veterinary Medicine, Hunan Agricultural University, Changsha, 410128, Hunan, China
2. Hunan Engineering Technology
Research Center of Veterinary Drugs, Hunan Agricultural University, Changsha, 410128, Hunan, China
Abstract
Aim:
The aim of this study was to investigate the metabolism of Gelsemium elegans in human, pig,
goat and rat liver microsomes and to elucidate the metabolic pathways and cleavage patterns of the Gelsemium
alkaloids among different species.
Methods:
A human, goat, pig and rat liver microsomes were incubated in vitro. After incubating at 37°C for 1
hour and centrifuging, the processed samples were detected by HPLC/Qq-TOFMS was used to detect alcohol
extract of Gelsemium elegans and its metabolites
Results:
Forty-six natural products were characterized from alcohol extract of Gelsemium elegans and 13
metabolites were identified. These 13 metabolites belong to the gelsemine, koumine, gelsedine, humantenine,
yohimbane, and sarpagine classes of alkaloids. The metabolic pathways included oxidation, demethylation and
dehydrogenation. After preliminary identification, the metabolites detected in the four species were different.
All 13 metabolites were detected in pig and rat microsomes, but no oxidative metabolites of Gelsedine-type alkaloids
were detected in goat and human microsomes.
Conclusion:
In this study, Gelsemium elegans metabolic patterns in different species are clarified and the in
vitro metabolism of Gelsemium elegans is investigated. It is of great significance for its clinical development
and rational application.
Funder
National Natural Science Foundation of China
Publisher
Bentham Science Publishers Ltd.