Affiliation:
1. Department of Dermatology, The Second Affiliated Hospital of Xi’an Jiaotong University, 157 Xiwu Road, Xi’an, 710004, China
2. Clinical Laboratory Center of Shaoxing People's Hospital (Shaoxing Hospital, Zhejiang University School of Medicine), Shaoxing,
China
Abstract
Background:
Circular RNA, as a member of noncoding RNA, plays an important role in the occurrence,
development and metastasis of tumor cells. So far, the correlation between circular RNA and malignant melanoma
remains obscure.
Methods:
RNA expression of circFAT1 and miR-375 in malignant melanoma (MM) tissues and cell lines was detected
by RT-PCR. The proliferation, cloning, migration and invasion of SK-Mel-28 and A375 cells were assessed using
CCK-8 test, clone formation and Transwell assay, respectively. CircRNA immunoprecipitation was used to validate the
relationship between circFAT1 and miR-375. The binding between circFAT1 and miR-375, as well as SLC7A11 and
miR-375 were verified by luciferase assay.
Results:
In our study，the circFAT1 was significantly overexpressed in the MM tissue than melanocytic nevi. Conversely,
the expression of miR-375 in MM tissue was lower than in melanocytic nevi tissue. The underexpression
of circFAT1 with siRNA plasmids significantly suppressed the proliferation, invasion and clone formation of MM cell
line. Mechanistically, circFAT1 positively regulates the expression level of SLC7A11 by sponging miR-375. The
promotive effects of circFAT1 on the proliferation and invasion ability of MM cells were reversed by the upregulation
of miR-375.
Conclusion:
circFAT1 promotes the proliferation, invasion and clone formation of malignant melanoma cells by improving
the expression level of SLC7A11 via sponging miR-375.
Funder
Zhejiang Medical Science and Technology Program
Publisher
Bentham Science Publishers Ltd.
Subject
Cancer Research,Pharmacology,Molecular Medicine