Affiliation:
1. State Key Laboratory of Hulless Barley and Yak Germplasm Resources and Genetic Improvement, Lhasa, China
2. Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Sichuan Province and Ministry of Education, Southwest Minzu University, Chengdu, China
Abstract
Background::
Mechanisms underlying yak adaptation to high-altitude environments have
been investigated at the levels of morphology, anatomy, physiology, genome and transcriptome, but
have not been explored at the proteome level.
Objective:
The protein profiles were compared between yak and cattle to explore molecular mechanisms
underlying yak adaptation to high altitude conditions.
Methods:
In the present study, an antibody microarray chip was developed, which included 6,500
mouse monoclonal antibodies. Immunoprecipitation and mass spectrometry were performed on 12
selected antibodies which showed that the chip was highly specific. Using this chip, muscle tissue proteome
was compared between yak and cattle, and 12 significantly Differentially Expressed Proteins (DEPs)
between yak and cattle were identified. Their expression levels were validated using Western blot.
Results:
ompared with cattle, higher levels of Rieske Iron-Sulfur Protein (RISP), Cytochrome C oxidase
subunit 4 isoform 1, mitochondrial (COX4I1), ATP synthase F1 subunit beta (ATP5F1B), Sarcoplasmic/
Endoplasmic Reticulum Calcium ATPase1 (SERCA1) and Adenosine Monophosphate Deaminase1
(AMPD1) in yak might improve oxygen utilization and energy metabolism. Pyruvate Dehydrogenase
protein X component (PDHX) and Acetyltransferase component of pyruvate dehydrogenase
complex (DLAT) showed higher expression levels and L-lactate dehydrogenase A chain (LDHA)
showed lower expression level in yak, which might help yak reduce the accumulation of lactic acid. In
addition, higher expression levels of Filamin C (FLNC) and low levels of AHNAK and Four and a half
LIM domains 1 (FHL1) in yak might reduce the risks of pulmonary arteries vasoconstriction, remodeling
and hypertension.
Conclusion:
Overall, the present study reported the differences in protein profile between yak and cattle,
which might be helpful to further understand molecular mechanisms underlying yak adaptation to
high altitude environments.
Funder
Tibet Academy of Agricultural and Animal Husbandry and Animal Husbandry Sciences
Basic Research Programs of Sichuan Province
National Beef Cattle and Yak Industrial Technology System
Provincial Department of Finance of the Tibet Autonomous Region
Publisher
Bentham Science Publishers Ltd.
Subject
Molecular Biology,Biochemistry
Cited by
2 articles.
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