Affiliation:
1. School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, Sichuan, China
2. Institute of Qinghai-Tibetan Plateau, Southwest Minzu University, Chengdu 610041, Sichuan, China
Abstract
<P>Background: Yaks inhabit high-altitude are well-adapted to the hypoxic environments.
Though, the mechanisms involved in regulatory myocardial protein expression at high-altitude were
not completely understood.
</P><P>
Objective: To revel the molecular mechanism of hypoxic adaptation in yak, here we have applied comparative
myocardial proteomics in between yak and cattle by isobaric Tag for Relative and Absolute
Quantitation (iTRAQ) labelling.
</P><P>
Methods: To understand the systematic protein expression variations in myocardial tissues that explain
the hypoxic adaptation in yak, we have performed iTRAQ analysis combined with Liquid Chromatography-
Tandem Mass Spectrometry (LC-MS/MS). Bioinformatics analysis was performed to find the
association of these Differentially Expressed Proteins (DEPs) in different functions and pathways. Protein
to protein interaction was analyzed by using STRING database.
</P><P>
Results: 686 Differentially Expressed Proteins (DEPs) were identified in yak with respect to cattle.
From which, 480 DEPs were up-regulated and 206 were down-regulated in yak. Upregulated expression
of ASB4, STAT, HRG, RHO and TSP4 in yak may be associated with angiogenesis, cardiovascular
development, response to pressure overload to heart and regulation of myocardial contraction in response
to increased oxygen tension. The up-regulation of mitochondrial proteins, ACAD8, GPDH-M,
PTPMT1, and ALDH2, may have contributed to oxidation within mitochondria, hypoxia-induced cell
metabolism and protection of heart against cardiac ischemic injuries. Further, the upregulated expression
of SAA1, PTX, HP and MBL2 involved in immune response potentially helpful in myocardial
protection against ischemic injuries, extracellular matrix remodeling and free heme neutralization/
clearance in oxygen-deficient environment.
</P><P>
Conclusion: Therefore, the identification of these myocardial proteins in will be conducive to investigation
of the molecular mechanisms involved in hypoxic adaptations of yaks at high-altitude condition.</P>
Funder
Longshan Academic Talent Research Supporting
Publisher
Bentham Science Publishers Ltd.
Subject
Molecular Biology,Biochemistry
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