Affiliation:
1. Jiangxi University of Traditional Chinese Medicine, Nanchang 330006, China
2. Guizhou Medcial University, Guiyang 550004, China
Abstract
Background:
Erding granule (EDG) widely used as an agent with the effect of heat-clearing,
detoxifying, eliminating dampness, relieving jaundice and upper respiratory tract disease in clinical
application, but the systematic chemical information and anti-hyperuricemia effect of EDG was still
unclear.
Methods:
An ultra-high performance liquid chromatography combined with electrospray ionization
quadrupole time-of-flight mass spectrometry (UHPLC/ESI-Q-TOF-MS/MS) method was utilized to
rapidly identify the chemical constituents of EDG. The anti-hyperuricemia effect of EDG was evaluated
based on the effect on xanthine oxidase inhibitory activity (in vitro) and lowering uric acid (in vivo).
Results:
198 compounds were tentatively separated and identified or characterized within 30 min by
UHPLC/ESI-Q-TOF MS/MS. These compounds were categorized as 22 coumarins, 38 flavones, 67
alkaloids, 36 organic acids, 16 sesquiterpenes, 14 lignans and 5 the others constituents. Meanwhile,
EDG significantly decreases the serum urate level of hyperuricemic mice induced by potassium
oxonate, while EDG did not significantly decrease the serum urate level of hyperuricemic mice induced
by hypoxanthine and activity of xanthine oxidase in vitro.
Conclusion:
The method developed was rapid and sensitive to characterize the chemical constituents of
EDG, and provide a systematic view of chemical information for EDG. Furthermore, we first discovered
the anti-hyperuricemia effect of EDG and it would further provide the reference for clarifying the
mechanism of EDG on lowering uric acid.
Funder
Jiangxi province with 5511 advantage scientific and technological innovation team
Jiangxi Provincial Department of Science and Technology
Science and technology research project of Jiangxi Provincial Education Department
National Natural Science Foundation of China
Publisher
Bentham Science Publishers Ltd.
Subject
Pharmaceutical Science,Molecular Medicine,Biochemistry,Biophysics
Cited by
1 articles.
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