Affiliation:
1. Department of Pharmaceutical Chemistry, JSS College of Pharmacy, Mysuru Karnataka 570015, India
Abstract
Background:
A normal phase- High Performance Liquid Chromatographic (HPLC) method
was developed for the enantioseparation of Oxomemazine.
Methods:
Separation of enantiomers was attained on Amylose Tris (5-chloro-2-methylphenylcarbamate)
using n-hexane: Iso-propyl Alcohol (IPA): Diethylamine (DEA) (60: 40: 0.1) as the
mobile phase and the peaks were observed at 227nm using PDA detector. The run time of the analysis
was set to 30 min.
Results:
Linearity was found in the range 10-50 μgmL-1. The enantiomers were separated at retention
times 16.87 min and 21.37 min.
Conclusion:
The developed method was validated as per the ICH guidelines, thus proving the method
to be selective, precise and showing linear response of Oxomemazine peak areas. Additionally, the
method of chiral separation was being understood by docking simulation study. The method was appropriate
for analysis of Oxomemazine in the pure form and its formulation.
Publisher
Bentham Science Publishers Ltd.
Subject
Pharmaceutical Science,Molecular Medicine,Biochemistry,Biophysics
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