Affiliation:
1. School of Life Sciences and Biotechnology, Kyungpook National University, Daegu 702-701, Korea
2. Department of Physiology, College of Medicine, Yeungnam University, Daegu 705-717, Korea
Abstract
Gene therapy is a powerful technology for treating incurable and hereditary disease in humans.
In the recent years, a lot of studies have been done on the development of DNA carriers. Nonviral
vectors, like liposomes, polymers, and micelles, has become common vehicles due to their safety.
The key compound of DOGS, DOSPA and DOSPER is the 5-carboxyspermine which formed the poly
amine head-group and carries four positive charges. In general, multivalent head-group shows more
transfection efficiency than monovalent analogues. In this paper, the efficient and simple synthesis of
Boc-protected 5-carboxyspermine is described. Boc-protected polyamines were synthesized from (S)-
2,5-diaminopentanoic acid monohydrochloride (L-ornithine) monohydrochloride through both cyanoethylation
and direct catalytic reduction of nitriles with the combination of nickel (II) chloride, sodium
borohydride and di-tert-butyl decarbonate (BOC2O) in a one-pot two-reaction.
Funder
Ministry of Education of Thailand
Publisher
Bentham Science Publishers Ltd.
Subject
Organic Chemistry,Biochemistry
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