Affiliation:
1. Department of Forensic Science, School of Basic Medical Science, Xiangya School of Medicine, Central South University, Changsha, Hunan, China
Abstract
Background:
The abuse of psychostimulants such as methamphetamine (METH) is
common in human immunodeficiency virus (HIV)-infected individuals. Acquired immunodeficiency
syndrome (AIDS) patients taking METH and antiretroviral drugs could suffer severe neurologic
damage and cognitive impairment.
Objective:
To reveal the underlying neuropathologic mechanisms of an HIV protease inhibitor (PI)
combined with METH, growth-inhibition tests of dopaminergic cells and RNA sequencing were performed.
Methods:
A combination of METH and PI caused more growth inhibition of dopaminergic cells
than METH alone or a PI alone. Furthermore, we identified differentially expressed gene (DEG) patterns
in the METH vs. untreated cells (1161 genes), PI vs. untreated cells (16 genes), METH-PI vs.
PI (3959 genes), and METH-PI vs. METH groups (14 genes).
Results:
The DEGs in the METH-PI co-treatment group were verified in the brains of a mouse
model using quantitative polymerase chain reaction and were involved mostly in the regulatory functions
of cell proliferation and inflammation.
Conclusion:
Such identification of key regulatory genes could facilitate the study of their neuroprotective
potential in the users of METH and PIs.
Funder
Fundamental Research Funds for the Central Universities of Central South University
Natural Science Foundation of Hunan Province, China
National Nature Science Foundation of China, China
Publisher
Bentham Science Publishers Ltd.
Subject
Virology,Infectious Diseases
Cited by
3 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献