Development and validation of stability-indicating RP-HPLC method for the estimation of azoxystrobin in its formulations

Abstract

The current work established and validated a simple, selective, precise, and accurate HighPerformance Liquid Chromatographic technique (HPLC) for the analysis of Azoxystrobin in its formulations. The mobile phase is made up of a combination of mobile phases comprising Acetonitrile and water in proportion, 80:20 (v/v). At a run duration of 15 minutes, this was found to yield a sharp peak of Azoxystrobin. Azoxystrobin was analysed using HPLC at a wavelength of 255 nm at a flow rate of 1.0 mL/min. The calibration curve's linear regression analysis results revealed a satisfactory linear connection with a regression coefficient of 0.999 in the concentration range of 50% to 150 %. The linear regression equation was y = 2025x +123.2.The proposed approach was used to analyse Azoxystrobin with a high degree of precision and accuracy.The method was validated for precision, accuracy, specificity, ruggedness and robustness. This method is useful for the quantification of Azoxystrobin because of its precision, accuracy, short retention duration, sensitivity, and mobile phase composition.