Affiliation:
1. All-Russian Plant Quarantine Center
Abstract
As part of monitoring works carried out by specialists of the All-Russian Plant Quarantine Center, samples of stone fruit crops collected in 20 Russian Federation subjects were tested by enzyme immunoassay for the presence of the following viruses: ACLSV, ApMV, APLPV, ArMV, CLRV, CRLV, PBNSPaV, PDV, PNRSV, PPV, PRMV, RpRSV, SLRSV, TBRV, ToRSV, and TRSV. The distribution of plum pox potyvirus (PPV), prunus necrotic ring spot ilarvirus (PNRSV), and prune dwarf ilarvirus (PDV) was determined across the area of 12, 12, and 8 Russian Federation subjects in 14.9 %, 11.9 %, and 1.7 % of test samples, respectively. The presence of these viruses was confirmed by polymerase chain reaction with subsequent sequencing of the amplification products obtained. The occurrence of these viruses was analyzed depending on the type of plantation, plant species, and region of distribution. The envelope protein gene region of 18 PNRSV isolates from five Russian Federation subjects was analyzed, which showed a significant genetic variability of this virus among the Russian population.
Publisher
Federal State Budgetary Scientific Institution All-Russian Horticultural Institute for Breeding Agrotechnology and Nursery
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