Screening methods to identify the complex of strains of Plum pox virus (PPV)

Abstract

From the 10 known strains of thePlumpox virus (PPV) in the Russian Federation 7 strains are spread – D, M. Rec, W, C, CV, and CR with the prevalence of D and W strains. A characteristic feature of the Plum pox virus population in the Russian Federation is the wide spread of W, C and CR strains, extremely rare or not found at all in other regions of the world. Screening tests for detection of the complex of PPV strains by enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) were carried out. It has been established that effective detection of PPV-D, PPV-M, PPV-Rec, PPV-C, PPV-CR, PPV-EA, PPV-W and PPV-T strains is achieved using PPV-ELISA test systems with polyclonal antibodies from ACD, Adgen, Agdia, Bioreba, DSMZ and Loewe companies. Monoclonal antibodies 5B-IVIA do not allow to diagnose isolates related to the strain PPV-CR. For universal detection of isolates of all PPV strains, it is necessary to use a real-time PCR kit for PPV (Syntol), a FLASH-PCR kit for PPV (Agrodiagnostica) or conventional PCR with one of the following primer pairs: P1/P2, s1/as2 or 3´NCP sense/3´NCP antisense.