Affiliation:
1. General University Hospital and First Faculty of Medicine, Prague, Czech Republic
2. National Museum, Prague, Czech Republic
Abstract
Comparison of 12 DNA extraction kits for vertebrate samples
Martincová, I. Aghová, T.
Abstract
Obtaining high quality DNA extractions is a crucial step for molecular biology research projects. At present, numerous protocols are available for vertebrate tissue extractions. In the present study we compared eleven column–based protocols and one HotSHOT protocol using similar conditions (i.e., type of sample, weight of starting material). We evaluated time of extraction, quality and quantity of DNA yield, and price of extraction for a single sample. Based on our analysis, the most successful kits for producing DNA with the highest concentration and purity are the JetQuick® Genomic DNA Purification Kit (Genomed) and the NucleoSpin® Tissue (Macherey–Nagel). Nevertheless, it is highly recommended to test various extraction kits with specific samples to find the optimal kit in all aspects of time, quality and cost for a particular project.
Publisher
Consorci del Museu de Ciencies Naturals de Barcelona
Subject
Nature and Landscape Conservation,Animal Science and Zoology
Cited by
4 articles.
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