STUDY OF ABSORBENT PROPERTIES OF FRESHWATER ALGAE LEMNA MINOR ACCORDING TO THE LEVEL OF IODINE ACCUMULATION

Abstract

The article describes the unique properties of algae to intensively build up biomass and accumulate the mineral elements present in it from the water environment, the justification of the direction of research on the sorption capacity of freshwater and marine algae, the scheme of the experiment, used materials and methods are described. The study of the sorption capacity of the freshwater algae Lemna minor was carried out in a vivarium under 24-hour lighting using fluorescent lamps, the ambient temperature was 28-30 °C, the air humidity was 75-80%, the temperature of the lake water in glass containers varied between 22-25 ºС. An equal mass of freshwater algae Lemna minor was placed in the prepared water medium with different concentrations of iodine in the form of potassium iodide. The content of iodine in wet and dry algae biomass was determined at the beginning of the experiment, after 15 days and at the end of the experiment. To obtain dry biomass, test samples with different concentrations of iodine were dried at a temperature of 85 ºС. To determine the content of iodine in watercress biomass, the capillary electrophoresis method was used using the Kapel-105M capillary electrophoresis system with a negative polarity high voltage source. In the course of the experiment, a significant increase in the iodine content in the dry biomass of Lemna minor was noted both after 15 days of cultivation and at the end of the experiment. Based on the results of studies of the sorption capacity of the biomass of the freshwater algae Lemna minor according to the level of iodine accumulation, the ability of this algae to accumulate iodine content in its biomass, depending on the concentration of iodine in the cultivation medium, was established. The highest intensity of iodine accumulation in duckweed biomass was noted when it was grown on cultivation media with an iodine concentration of 40-2000 mg/dm3. High concentrations of Iodine in the culture medium had a negative effect on the sorption properties of Lemna minor, accumulation of Iodine in it, delayed growth, and by the end of the experiment caused its death.