Dispersion liquid microextraction of fluoroquinolones

Abstract

The dispersive liquid-liquid microextraction method of norfloxacin, ciprofloxacin and enrofloxacin with chromatographic detection was developed. The optimization of dispersive microextraction was carried out, and the optimal parameters were determined as follows: 5 ml of aqueous solution; 70 l of chloroform; 500 l of isopropanol; extraction time of 5 min; centrifugation time of 5 min; and pH 7.0–9.0. The concentration range was 4.0–100 g/ml, and the limit of detection was not exceeded 1.5 g/ml. The microextraction method was tested on mineral water samples by the standard addition method. The obtained results indicate sufficient accuracy and reproducibility of the developed method. The relative standard deviation does not exceed 2%.