Dual receptors blocked mechanism arbitrates smooth muscles relaxant effect of Polypodium vulgare

Abstract

<p class="Abstract">The study was aimed to validate the traditional uses of <em>Polypodium vulgare</em> in disorders associated with smooth muscle contraction and to describe its possible underlying mechanism(s) by using <em>in vitro</em> and <em>in vivo</em> experimental techniques. Rhizome’s extract of <em>P. vulgare</em> reversed the high K⁺(80 mM) and carbachol (1 µM) mediated contractions in isolated rabbit jejunum (5 and 10 mg/mL), trachea (5 and 10 mg/mL) and urinary bladder (3 and 10 mg/mL), with higher potency against carbachol than high K⁺, similar to dicyclomine. A rightward shift in carbachol cumulative response curves was observed in the presence of crude extract (1-3 mg/mL), similar to dicyclomine. Crude extract exhibited a dose-dependent (300-500 mg/kg) protective effect against castor oil-induced diarrhea in mice. Presence of dual blacked mechanism behind the smooth muscles relaxant effect of the crude extract, unveil medicinal significance of <em>P. vulgare</em> in GIT, respiratory and urinary bladder disorders.</p><p class="Abstract"><strong>Video clip</strong></p><p class="Abstract"><a href="https://youtube.com/v/Ya9Rhtlg3bE">Tissue preparation</a>: 3 min 5 sec</p><p> </p>