Genome-Wide Analysis of Spatial Gene Expression in Arabidopsis Flowers[W]
Author:
Wellmer Frank1, Riechmann Joseé Luis1, Alves-Ferreira Maércio1, Meyerowitz Elliot M.
Affiliation:
1. California Institute of Technology, Division of Biology 156-29, Pasadena, California 91125
Abstract
Abstract
We have compared the gene expression profiles of inflorescences of the floral homeotic mutants apetala1, apetala2, apetala3, pistillata, and agamous with that of wild-type plants using a flower-specific cDNA microarray and a whole genome oligonucleotide array. By combining the data sets from the individual mutant/wild type comparisons, we were able to identify a large number of genes that are, within flowers, predicted to be specifically or at least predominantly expressed in one type of floral organ. We have analyzed the expression patterns of several of these genes by in situ hybridization and found that they match the predictions that were made based on the microarray experiments. Moreover, genes with known floral organ–specific expression patterns were correctly assigned by our analysis. The vast majority of the identified transcripts are found in stamens or carpels, whereas few genes are predicted to be expressed specifically or predominantly in sepals or petals. These findings indicate that spatially limited expression of a large number of genes is part of flower development and that its extent differs significantly between the reproductive organs and the organs of the perianth.
Publisher
Oxford University Press (OUP)
Subject
Cell Biology,Plant Science
Reference71 articles.
1. Aarts, M.G., Hodge, R., Kalantidis, K., Florack, D., Wilson, Z.A., Mulligan, B.J., Stiekema, W.J., Scott, R., and Pereira, A. (1997). The Arabidopsis MALE STERILITY 2 protein shares similarity with reductases in elongation/condensation complexes. Plant J. 12
, 615–623. 2. Alonso, J.M., et al. (2003). Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301
, 653–657. 3. Altschul, S.F., Gish, W., Miller, W., Myers, E.W., and Lipman, D.J. (1990). Basic local alignment search tool. J. Mol. Biol. 215
, 403–410. 4. Alves-Ferreira, M., Engler, J.A., Miguens, F.C., Van Montagu, M., Engler, G., and Oliveira, D.E. (1997). Oleosin gene expression in Arabidopsis thaliana coincides with accumulation of lipid in plastids and in cytoplasmatic bodies. Plant Physiol. Biochem. 35
, 729–739. 5. Asamizu, E., Nakamura, Y., Sato, S., and Tabata, S. (2000). A large scale analysis of cDNA in Arabidopsis thaliana: Generation of 12,028 non-redundant expressed sequence tags from normalized and size-selected cDNA libraries. DNA Res. 7
, 175–180.
Cited by
211 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献
|
|