Genetic diversity of multidrug resistant Salmonella enterica subsp. enterica serovar Brancaster isolated from chicken in Malaysia
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Published:2023-11-13
Issue:
Volume:
Page:9-20
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ISSN:2672-7277
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Container-title:Asia Pacific Journal of Molecular Biology and Biotechnology
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language:en
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Short-container-title:APJMBB
Author:
Khoo Evie1, Roslee Roseliza2, Zakaria Zunita1, Ahmad Nur Indah1
Affiliation:
1. Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia 2. Bacteriology Section, Veterinary Research Institute, 59 Jalan Sultan Azlan Shah, 31400 Ipoh, Perak, Malaysia
Abstract
Salmonella enterica subsp. enterica serovar Brancaster is a chicken-associated serovar that is increasingly reported in the Asian region, including Malaysia. The shortfall of conventional serovar identification for nomenclature alone is insufficient for studying the genetic and clonal relationships of Salmonella of the same serovar. Whereas DNA sequence-based typing method is useful to study the genetic diversity and relatedness of the highly diverse Salmonella. In this study, the genetic diversity, phenotypic antimicrobial resistance (AMR) profiles, and detection of selected AMR genes from ten representative Salmonella Brancaster isolates from chicken cloacal swabs and raw chicken meat were carried out. Based on multilocus sequence typing (MLST), all Salmonella Brancaster belonged to sequence type ST2133. Further analysis of the MLST sequencing data using concatenated nucleotide sequences of the seven housekeeping genes divided them into nine clusters, revealed heterogeneity and genetic diversity among the isolates. All isolates were multidrug-resistant (MDR), which confers resistance to three or more classes of antibiotics. Seven Salmonella Brancaster isolates exhibited phenotypic ACSSuT-type MDR profile, and two of them were also resistant towards ciprofloxacin. Salmonella Brancaster in the present study possessed at least one and up to six resistance genes, with the most prevalent being the ampicillin (blaTEM), tetracycline (tetA), chloramphenicol (floR), gentamicin (ant(3”)-Ia), streptomycin (strA), and sulfonamides (sul-2). Chickens are common reservoir for serovar Brancaster and the emergence, persistence, and dissemination of MDR Salmonella Brancaster possessed a public health risk. The authorities need to strengthen salmonellosis control management programme in poultry farms, abattoirs, and processing facilities.
Funder
Universiti Putra Malaysia
Publisher
Malaysian Society for Molecular Biology and Biotechnology
Subject
Molecular Biology,Biotechnology
Reference42 articles.
1. Abatcha, M. G., Effarizah, M. E., & Rusul, G. 2018. Prevalence, antimicrobial resistance, resistance genes and class 1 integrons of Salmonella serovars in leafy vegetables, chicken carcasses and related processing environments in Malaysian fresh food markets. Food Control 91: 170–180. 2. Abdullah, N. B. A., & Ismail, A. F. 2021. Food poisoning outbreaks among schoolchildren in Terengganu and their associated factors. Sains Malaysiana 50(4): 1027–1036. 3. Bacci, C., Boni, E., Alpigiani, I., Lanzoni, E., Bonardi, S., & Brindani, F. 2012. Phenotypic and genotypic features of antibiotic resistance in Salmonella enterica isolated from chicken meat and chicken and quail carcasses. International Journal of Food Microbiology 160: 16–23. 4. Bell, R. L., González-Escalona, N., Stones, R., & Brown, E. W. 2011. Phylogenetic evaluation of the ‘Typhimurium’ complex of Salmonella strains using a seven-gene multi-locus sequence analysis. Infection, Genetics and Evolution 11: 83–91. 5. Cardinale, E., Gros-Claude, J. D. P., Rivoal, K., Rose, V., Tall, F., Mead, G. C., & Salvat, G. 2005. Epidemiological analysis of Salmonella enterica ssp. enterica serovars Hadar, Brancaster and Enteritidis from humans and broiler chickens in Senegal using pulsed-field gel electrophoresis and antibiotic susceptibility. Journal of Applied Microbiology 99: 968–977.
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