An Interscholastic Network To Generate LexA Enhancer Trap Lines in Drosophila
Author:
Kockel Lutz1, Griffin Catherine2, Ahmed Yaseen2, Fidelak Lauren2, Rajan Arjun2, Gould Ethan P2, Haigney Myles2, Ralston Benjamin2, Tercek Rex J2, Galligani Lara2, Rao Sagar2, Huq Lutfi2, Bhargava Hersh K2, Dooner Ailis C2, Lemmerman Emily G2, Malusa Ruby F2, Nguyen Tran H2, Chung Julie S2, Gregory Sara M2, Kuwana Kiyomasa M2, Regenold Jonathan T2, Wei Alexander2, Ashton Jake2, Dickinson Patrick2, Martel Kate2, Cai Connie2, Chen Carissa2, Price Stephen2, Qiao Jeffrey2, Shepley David2, Zhang Joanna2, Chalasani Meghana2, Nguyen Khanh2, Aalto August2, Kim ByungJun2, Tazawa-Goodchild Erik2, Sherwood Amanda2, Rahman Ahmad2, Wu Sum Ying Celeste2, Lotzkar Joel2, Michaels Serena2, Aristotle Hillary2, Clark Antigone2, Gasper Grace2, Xiang Evan2, Schlör Frieda Luna2, Lu Melissa2, Haering Kate2, Friberg Julia2, Kuwana Alyssa2, Lee Jonathan2, Liu Alan2, Norton Emma2, Hamad Leena2, Lee Clara2, Okeremi Dara2, diTullio Harry2, Dumoulin Kat2, Chi Sun Yu Gordon2, Derossi Grayson S2, Horowitch Rose E2, Issa Elias C2, Le Dan T2, Morales Bryce C2, Noori Ayush2, Shao Justin2, Cho Sophia2, Hoang Mai N2, Johnson Ian M2, Lee Katherine C2, Lee Maria2, Madamidola Elizabeth A2, Schmitt Katrina E2, Byan Gabriel2, Park Taeyoung2, Chen Jonathan2, Monovoukas Alexi2, Kang Madison J2, McGowan Tanner2, Walewski Joseph J2, Simon Brennan2, Zu Sophia J2, Miller Gregory P2, Fitzpatrick Kate B2, Lantz Nicole3, Fox Elizabeth3, Collette Jeanette4, Kurtz Richard4, Duncan Chris5, Palmer Ryan5, Rotondo Cheryl6, Janicki Eric6, Chisholm Townley6, Rankin Anne6, Park Sangbin1, Kim Seung K178
Affiliation:
1. Dept. of Developmental Biology, Stanford University School of Medicine, Stanford CA 94305 2. Phillips Exeter Academy, Exeter, NH 03833 3. The Lawrenceville School, 2500 Main St, NJ 08648 4. Commack High School, 1 Scholar Ln, Commack, NY 11725 5. Pritzker College Prep, 4131 W Cortland St, Chicago, IL 60639 6. Science Department, Phillips Exeter Academy, Exeter, NH 03833 7. Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305 8. Stanford Diabetes Research Center, Stanford, CA 94305
Abstract
Abstract
Binary expression systems like the LexA-LexAop system provide a powerful experimental tool kit to study gene and tissue function in developmental biology, neurobiology, and physiology. However, the number of well-defined LexA enhancer trap insertions remains limited. In this study, we present the molecular characterization and initial tissue expression analysis of nearly 100 novel StanEx LexA enhancer traps, derived from the StanEx1 index line. This includes 76 insertions into novel, distinct gene loci not previously associated with enhancer traps or targeted LexA constructs. Additionally, our studies revealed evidence for selective transposase-dependent replacement of a previously-undetected KP element on chromosome III within the StanEx1 genetic background during hybrid dysgenesis, suggesting a molecular basis for the over-representation of LexA insertions at the NK7.1 locus in our screen. Production and characterization of novel fly lines were performed by students and teachers in experiment-based genetics classes within a geographically diverse network of public and independent high schools. Thus, unique partnerships between secondary schools and university-based programs have produced and characterized novel genetic and molecular resources in Drosophila for open-source distribution, and provide paradigms for development of science education through experience-based pedagogy.
Publisher
Oxford University Press (OUP)
Subject
Genetics (clinical),Genetics,Molecular Biology
Cited by
11 articles.
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