Characterization of Interactions Between and Among Components of the Meiotic Silencing by Unpaired DNA Machinery in Neurospora crassa Using Bimolecular Fluorescence Complementation-Reference-Cited by-同舟云学术

Characterization of Interactions Between and Among Components of the Meiotic Silencing by Unpaired DNA Machinery in Neurospora crassa Using Bimolecular Fluorescence Complementation

Published:2008-01-01 Issue:1 Volume:178 Page:593-596
ISSN:1943-2631
Container-title:Genetics
language:en
Short-container-title:

Author:

Bardiya Nirmala¹,Alexander William G¹,Perdue Tony D²,Barry Edward G²,Metzenberg Robert L³,Pukkila Patricia J²,Shiu Patrick K T¹

Affiliation:

1. Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211

2. Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599 and

3. Department of Biology, California State University, Northridge, California 91330

Abstract

Abstract Bimolecular fluorescence complementation (BiFC) is based on the complementation between two nonfluorescent fragments of the yellow fluorescent protein (YFP) when they are united by interactions between proteins covalently linked to them. We have successfully applied BiFC in Neurospora crassa using two genes involved in meiotic silencing by unpaired DNA (MSUD) and observed macromolecular complex formation involving only SAD-1 proteins, only SAD-2 proteins, and mixtures of SAD-1 and SAD-2 proteins.

Publisher

Oxford University Press (OUP)

Subject

Genetics

Link

https://academic.oup.com/genetics/article-pdf/178/1/593/42094515/genetics0593.pdf

Reference19 articles.

1. GFP as a tool to analyze the organization, dynamics and function of nuclei and microtubules in Neurospora crassa

2. Use of bimolecular fluorescence complementation to demonstrate transcription factor interaction in nuclei of living cells from the filamentous fungus Acremonium chrysogenum

3. Visualization of Interactions among bZIP and Rel Family Proteins in Living Cells Using Bimolecular Fluorescence Complementation

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