Affiliation:
1. Division of Biological Sciences, University of Missouri , Columbia, MO 65211 , USA
2. School of Biological Sciences, Illinois State University , Normal, IL 61790 , USA
Abstract
Abstract
During the sexual phase of Neurospora crassa, unpaired genes are subject to a silencing mechanism known as meiotic silencing by unpaired DNA (MSUD). MSUD targets the transcripts of an unpaired gene and utilizes typical RNA interference factors for its process. Using a reverse genetic screen, we have identified a meiotic silencing gene called sad-9, which encodes a DEAD-box RNA helicase. While not essential for vegetative growth, SAD-9 plays a crucial role in both sexual development and MSUD. Our results suggest that SAD-9, with the help of the SAD-2 scaffold protein, recruits the SMS-2 Argonaute to the perinuclear region, the center of MSUD activity.
Funder
National Institute of General Medical Sciences
Graduate Assistance in Areas of National Need Fellowship
US Department of Education
Federal Work-Study Program
Ruth L. Kirschstein National Research Service Award
MU Research Board
MU Research Council
National Science Foundation
Publisher
Oxford University Press (OUP)
Subject
Genetics (clinical),Genetics,Molecular Biology
Cited by
1 articles.
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