Genome Engineering of Drosophila with the CRISPR RNA-Guided Cas9 Nuclease-Reference-Cited by-同舟云学术

Genome Engineering of Drosophila with the CRISPR RNA-Guided Cas9 Nuclease

Published:2013-08-01 Issue:4 Volume:194 Page:1029-1035
ISSN:1943-2631
Container-title:Genetics
language:en
Short-container-title:

Author:

Gratz Scott J¹,Cummings Alexander M²,Nguyen Jennifer N³,Hamm Danielle C⁴,Donohue Laura K²,Harrison Melissa M⁴⁵,Wildonger Jill³,O’Connor-Giles Kate M¹²⁶

Affiliation:

1. Genetics Training Program, University of Wisconsin, Madison, Madison, Wisconsin 53706

2. Laboratory of Genetics, University of Wisconsin, Madison, Madison, Wisconsin 53706

3. Department of Biochemistry, University of Wisconsin, Madison, Madison, Wisconsin 53706

4. Integrated Program in Biochemistry, University of Wisconsin, Madison, Madison, Wisconsin 53706

5. Department of Biomolecular Chemistry, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin 53706

6. Laboratory of Cell and Molecular Biology, University of Wisconsin, Madison, Madison, Wisconsin 53706

Abstract

Abstract We have adapted a bacterial CRISPR RNA/Cas9 system to precisely engineer the Drosophila genome and report that Cas9-mediated genomic modifications are efficiently transmitted through the germline. This RNA-guided Cas9 system can be rapidly programmed to generate targeted alleles for probing gene function in Drosophila.

Publisher

Oxford University Press (OUP)

Subject

Genetics

Link

https://academic.oup.com/genetics/article-pdf/194/4/1029/42116546/genetics1029.pdf

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