Age-Specific Variation in Immune Response in Drosophila melanogaster Has a Genetic Basis

Author:

Felix Tashauna M1,Hughes Kimberly A2,Stone Eric A3,Drnevich Jenny M4,Leips Jeff1

Affiliation:

1. Department of Biological Sciences, University of Maryland, Baltimore, Maryland 21250

2. Department of Biological Science, Florida State University, Tallahassee, Florida 32306

3. Bioinformatics Research Center and Department of Genetics, North Carolina State University, Raleigh, North Carolina 27695, and

4. High Performance Biological Computing Group Roy J. Carver Biotechnology Center University of Illinois at Urbana-Champaign, Urbana, IL 61801

Abstract

Abstract Immunosenescence, the age-related decline in immune system function, is a general hallmark of aging. While much is known about the cellular and physiological changes that accompany immunosenescence, we know little about the genetic influences on this phenomenon. In this study we combined age-specific measurements of bacterial clearance ability following infection with whole-genome measurements of the transcriptional response to infection and wounding to identify genes that contribute to the natural variation in immunosenescence, using Drosophila melanogaster as a model system. Twenty inbred lines derived from nature were measured for their ability to clear an Escherichia coli infection at 1 and 4 weeks of age. We used microarrays to simultaneously determine genome-wide expression profiles in infected and wounded flies at each age for 12 of these lines. Lines exhibited significant genetically based variation in bacterial clearance at both ages; however, the genetic basis of this variation changed dramatically with age. Variation in gene expression was significantly correlated with bacterial clearance ability only in the older age group. At 4 weeks of age variation in the expression of 247 genes following infection was associated with genetic variation in bacterial clearance. Functional annotation analyses implicate genes involved in energy metabolism including those in the insulin signaling/TOR pathway as having significant associations with bacterial clearance in older individuals. Given the evolutionary conservation of the genes involved in energy metabolism, our results could have important implications for understanding immunosenescence in other organisms, including humans.

Publisher

Oxford University Press (OUP)

Subject

Genetics

Reference122 articles.

1. Investigating the involvement of host factors involved in intracellular pathogen infection by RNAi in Drosophila cells;Agaisse;Methods Mol. Biol.,2008

2. Altered innate immune functioning of dendritic cells in elderly humans: a role of phosphoinositide 3-kinase-signaling pathway;Agrawal;J. Immunol.,2007

3. Age-associated impaired plasmacytoid dendritic cell functions lead to decreased CD4 and CD8 T cell immunity;Agrawal;Age (Omaha),2011

4. Molecular evolution of the toll-like receptor multigene family in birds;Alcaide;Mol. Biol. Evol.,2011

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