Synergistic actions of three MYB transcription factors underpins the high accumulation of myricetin in Morella rubra

Abstract

SUMMARYFlavonols are health‐promoting bioactive compounds important for human nutrition, health, and plant defense. The transcriptional regulation of kaempferol and quercetin biosynthesis has been studied extensively, while little is known about the regulatory mechanisms underlying myricetin biosynthesis, which has strong antioxidant, anticancer, antidiabetic, and anti‐inflammatory activities. In this study, the flavonol‐specific MrMYB12 in Morella rubra preferred activating the promoter of flavonol synthase 2 (MrFLS2) (6.4‐fold) rather than MrFLS1 (1.4‐fold) and upregulated quercetin biosynthesis. Furthermore, two SG44 R2R3‐MYB members, MrMYB5 and MrMYB5L, were identified by yeast one‐hybrid library screening using the promoter of flavonoid 3′,5′‐hydroxylase (MrF3′5′H), and transcript levels of these R2R3‐MYBs were correlated with accumulation of myricetin derivatives during leaf development. Dual‐luciferase and electrophoretic mobility shift assays demonstrated that both MrMYB5 and MrMYB5L could bind directly to MYB recognition sequence elements in promoters of MrF3′5′H or MrFLS1 and activate their expression. Protein–protein interactions of MrMYB5 or MrMYB5L with MrbHLH2 were confirmed by yeast two‐hybrid and bimolecular fluorescence complementation assays. MrMYB5L‐MrbHLH2 showed much higher synergistic activation of MrF3′5′H or MrFLS1 promoters than MrMYB5‐MrbHLH2. Studies with Arabidopsis thaliana homologs AtMYB5 and AtTT8 indicated that similar synergistic regulatory effects occur with promoters of MrF3′5′H or MrFLS1. Transient overexpression of MrMYB5L‐MrbHLH2 in Nicotiana benthamiana induced a higher accumulation of myricetin derivatives (57.70 μg g−1 FW) than MrMYB5‐MrbHLH2 (7.43 μg g−1 FW) when MrMYB12 was coexpressed with them. This study reveals a novel transcriptional mechanism regulating myricetin biosynthesis with the potential use for future metabolic engineering of health‐promoting flavonols.