Expansion microscopy: A revolution in diagnostic pathology

Author:

Ghosh Snehashish1,Sapkota Bhawana Subedi2,Rao Roopa S3,Patil Shankargouda45,Rajkumar Chandini6,Lakshminarayan Surendra7

Affiliation:

1. Department of Oral Pathology College of Medical Sciences Bharatpur Nepal

2. Dental Officer College of Medical Sciences Bharatpur Nepal

3. Faculty of Dental Sciences Department of Oral Pathology, Ramaiah University of Applied Sciences Bangalore India

4. College of Dental Medicine Roseman University of Health Sciences, South Jordan, Utah

5. Centre of Molecular Medicine and Diagnostics (COMManD), Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University Chennai India

6. Department of Oral Pathology and Microbiology Sathyabama Dental College and Hospital Chennai India

7. Department of Oral Pathology and Microbiology, Faculty of Dental Sciences Ramaiah University of Applied Sciences Bengaluru India

Abstract

AbstractExpansion microscopy (ExM) is a recent discovery in diagnostic pathology and microbiology that promotes a physical magnification of the tissue specimen instead of optical magnification. It not only improves the resolution of the specimen but also enhances the diagnostic precision, and permits nanoscale imaging of the specimen. Optical microscopy is routinely used in histopathology and microbiology for ages. Due to its simplicity, compatibility with different types of specimens, and ease of operation it is accepted by pathologists. ExM and its variants have been widely tested in different types of tissue specimens and microbiological specimens and yielded brilliant results.

Publisher

Wiley

Subject

Histology,Pathology and Forensic Medicine

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