Affiliation:
1. Department of Physics – Enrico Fermi University of Pisa Largo Pontecorvo, 3 Pisa 56127 Italy
2. Department of Applied Physics University of Barcelona C/Martí i Franquès, 1 Barcelona 08028 Spain
3. Department of Physics – Emilio Segrè University of Palermo Viale delle Scienze, 18 Palermo 90128 Italy
Abstract
AbstractExpansion Microscopy (ExM) and Light‐Sheet Fluorescence Microscopy (LSFM) are forefront imaging techniques that enable high‐resolution visualization of biological specimens. ExM enhances nanoscale investigation using conventional fluorescence microscopes, while LSFM offers rapid, minimally invasive imaging over large volumes. This review explores the joint advancements of ExM and LSFM, focusing on the excellent performance of the integrated modality obtained from the combination of the two, which is refer to as ExLSFM. In doing so, the chemical processes required for ExM, the tailored optical setup of LSFM for examining expanded samples, and the adjustments in sample preparation for accurate data collection are emphasized. It is delve into various specimen types studied using this integrated method and assess its potential for future applications. The goal of this literature review is to enrich the comprehension of ExM and LSFM, encouraging their wider use and ongoing development, looking forward to the upcoming challenges, and anticipating innovations in these imaging techniques.