Affiliation:
1. Department of Animal Production and Preventive Veterinary Medicine School of Veterinary Medicine and Animal Sciences, São Paulo State University-UNESP Botucatu SP Brazil
2. Department of Animal Biology Biology Institute, University of Campinas-UNICAMP Campinas SP Brazil
3. Department of Genetics, Evolution, Microbiology and Immunology UNICAMP Campinas SP Brazil
4. Department of Microbiology and Immunology UNESP Botucatu SP Brazil
Abstract
Abstract
Diptera (Insecta) are able to transmit approximately 200 pathogenic microorganisms to humans and animals, causing more than 65 diseases, including bovine mastitis, which constitutes a major cause of economic losses in the dairy industry. In this scenario, 217 adult specimens of Diptera were collected from nine farms and identified to the family and/or species level. Of the 11 families recorded, Muscidae was the most prevalent (152 out of 217; 70%). All Diptera specimens were subjected to microbiological culture using conventional and selective media, and isolates were then identified at the species level by mass spectrometry. In total, 275 microorganisms were identified, with a predominance of pathogens related to environmental bovine mastitis (166/275 = 60·4%), that is, Enterococcus species (70/275 = 25·4%) and Escherichia coli (49/275 = 17·8%). Nontraditional agents related to bovine mastitis (called miscellaneous) were detected in 28% (77/275), as well as microorganisms with well-known zoonotic behaviour (e.g. Bacillus cereus). This is study contributes with knowledge of diversity of microorganisms carried by Diptera in the dairy environment, including pathogens associated with environmental and contagious bovine mastitis, and agents with human relevance. To our knowledge, a three-part chromogenic selective medium used to microbial culture of milk on farms was used for the first time to identification of pathogens in Diptera.
Funder
(Conselho Nacional de Desenvolvimento Científico e Tecnológico, CNPq
Fundação de Amparo à Pesquisa do Estado de São Paulo - FAPESP
Publisher
Oxford University Press (OUP)
Subject
Applied Microbiology and Biotechnology
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