Flies (Insecta, Diptera) collected in the environment of dairy farms as carriers of Rotavirus A and betacoronavirus-Reference-Cited by-同舟云学术

Flies (Insecta, Diptera) collected in the environment of dairy farms as carriers of Rotavirus A and betacoronavirus

Published:2023-02-01 Issue:3 Volume:134 Page:
ISSN:1365-2672
Container-title:Journal of Applied Microbiology
language:en
Short-container-title:

Author:

Bertolini Amanda Bezerra¹^ORCID,Thyssen Patrícia Jacqueline²^ORCID,Brandão Paulo Eduardo³,Prado Aline Marrara²,Silva Sheila Oliveira de Souza³,Mioni Mateus de Souza Ribeiro¹,de Gouvea Fábio Lucas Rezende¹,Pantoja José Carlos de Figueiredo¹,Langoni Helio¹,Guimarães Felipe Freitas¹,Joaquim Sâmea Fernandes¹,Guerra Simony Trevizan¹,Leite Domingos da Silva⁴,Rall Vera Mores⁵,Hernandes Rodrigo Tavanelli⁵,Lucheis Simone Baldini¹,Rossi Gabriel Augusto Marques⁶^ORCID,Ribeiro Márcio Garcia¹

Affiliation:

1. Department of Animal Production and Preventive Veterinary Medicine, School of Veterinary Medicine and Animal Sciences, São Paulo State University-UNESP , 18618-681 Botucatu, SP , Brazil

2. Department of Animal Biology, Institute of Biology, University of Campinas-UNICAMP, Campinas , 13083-862 SP , Brazil

3. Department of Preventive Veterinary Medicine and Animal Health, School of Veterinary Medicine and Animal Science, University of São Paulo, FMVZ-USP , 05508-900 São Paulo, SP , Brazil

4. Department of Genetics, Evolution, Microbiology, and Immunology, UNICAMP , 13083-862 Campinas, SP , Brazil

5. Department of Chemical and Biological Sciences, Institute of Biosciences, UNESP , 18618-689 Botucatu, SP , Brazil

6. Vila Velha University (UVV), Faculty of Veterinary Medicine, Department of Veterinary Medicine , 29102-920 Vila Velha, ES , Brazil

Abstract

AbstractAimsWe aimed to investigate the prevalence of rotavirus and coronavirus in dipterans that commonly inhabit the environment of dairy farms.Methods and ResultsWe collected 217 insect specimens from nine dairy farms, which were examined through hemi-nested RT-PCR followed by Sanger sequencing in search of VP1 and N genes for rotavirus and bovine coronavirus-BCoV, respectively. With a predominance of Muscidae (152/217 = 70%) 11 families of Diptera were identified. Rotavirus A (RVA) and betacoronavirus (BCoV) were detected in 14.7% (32/217) and 4.6% (10/217) of the dipterans, respectively. Sequencing of the amplicons was possible for 11.5% (25/217) of RVA and 0.5% (1/217) of BCoV, confirming the presence of these pathogens.ConclusionsOur findings highlight the role of dipterans as carriers of RVA and BCoV of great relevance for public and animal health.

Funder

FAPESP

Publisher

Oxford University Press (OUP)

Subject

Applied Microbiology and Biotechnology,General Medicine,Biotechnology

Link

https://academic.oup.com/jambio/advance-article-pdf/doi/10.1093/jambio/lxad020/49175501/lxad020.pdf

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