Automation aided optimization of cloning, expression and purification of enzymes of the bacterial sialic acid catabolic and sialylation pathways enzymes for structural studies

Author:

Bairy Sneha1,Gopalan Lakshmi Narayanan2,Setty Thanuja Gangi34,Srinivasachari Sathya3,Manjunath Lavanyaa35,Kumar Jay Prakash34,Guntupalli Sai R35,Bose Sucharita3,Nayak Vinod3,Ghosh Swagatha3,Sathyanarayanan Nitish34,Caing-Carlsson Rhawnie6,Wahlgren Weixiao Yuan67,Friemann Rosmarie67,Ramaswamy S.3,Neerathilingam Muniasamy12

Affiliation:

1. Centre for Cellular and Molecular Platforms; NCBS-TIFR; GKVK Campus Bellary Road Bangalore 560065 Karnataka India

2. Department of Lipid Science; CSIR-Central Food Technology and Research Institute; Mysuru 570020 Karnataka India

3. Institute for Stem Cell Biology and Regenerative Medicine; GKVK Campus Bellary Road Bangalore 560065 Karnataka India

4. The Institute of TransDisciplinary Health Sciences & Technology (TDU); Bengaluru Karnataka India

5. Manipal Academy of Higher Education; Manipal Karnataka India-576104

6. Department of Chemistry and Molecular Biology; University of Gothenburg; Box 462 Gothenburg S-40530 Sweden

7. Centre for Antibiotic Resistance Research (CARe) at University of Gothenburg; Box 440 S-40530 Gothenburg Sweden

Funder

VINNOVA

Department of Biotechnology , Ministry of Science and Technology

Vetenskapsrådet

Svenska Forskningsrådet Formas

Publisher

Wiley

Subject

Applied Microbiology and Biotechnology,Biochemistry,Bioengineering,Biotechnology

Reference19 articles.

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