Design and validation of a RT-qPCR procedure for diagnosis and quantification of most types of infectious pancreatic necrosis virus using a single pair of degenerated primers

Author:

Vázquez D1,Cutrín J M1,Olveira J G1,Dopazo C P1

Affiliation:

1. Departamento de Microbiología y Parasitología; Instituto de Acuicultura; Universidade de Santiago de Compostela; Santiago de Compostela Spain

Publisher

Wiley

Subject

Veterinary (miscalleneous),Aquatic Science

Reference26 articles.

1. Anonimous 2004 Guide to Performing Relative Quantitation of Gene Expression Using Real-Time Quantitative PCR http://www.gu.se/digitalAssets/1125/1125331_ABI_-_Guide_Relative_Quantification_using_realtime_PCR.pdf

2. Amplification efficiency of thermostable DNA polymerases;Arezi;Analytical Biochemistry,2003

3. Development and validation of a short-time cell culture and multiplex reverse transcriptase polymerase chain reaction assay for infectious pancreatic necrosis virus in Mexican farm-sampled rainbow trout;Barrera-Mejía;Journal of Aquatic Animal Health,2009

4. Effect of template on generating a standard curve for absolute quantification of an RNA virus by real-time reverse transcriptase-polymerase chain reaction;Bowers;Molecular and Cellular Probes,2011

5. Detection and quantitation of infectious pancreatic necrosis virus by real-time reverse transcriptase-polymerase chain reaction using lethal and non-lethal tissue sampling;Bowers;Journal of Virological Methods,2008

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