Design and validation of a RT-qPCR procedure for diagnosis and quantification of most types of infectious pancreatic necrosis virus using a single pair of degenerated primers
Author:
Affiliation:
1. Departamento de Microbiología y Parasitología; Instituto de Acuicultura; Universidade de Santiago de Compostela; Santiago de Compostela Spain
Publisher
Wiley
Subject
Veterinary (miscalleneous),Aquatic Science
Link
http://onlinelibrary.wiley.com/wol1/doi/10.1111/jfd.12590/fullpdf
Reference26 articles.
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2. Amplification efficiency of thermostable DNA polymerases;Arezi;Analytical Biochemistry,2003
3. Development and validation of a short-time cell culture and multiplex reverse transcriptase polymerase chain reaction assay for infectious pancreatic necrosis virus in Mexican farm-sampled rainbow trout;Barrera-Mejía;Journal of Aquatic Animal Health,2009
4. Effect of template on generating a standard curve for absolute quantification of an RNA virus by real-time reverse transcriptase-polymerase chain reaction;Bowers;Molecular and Cellular Probes,2011
5. Detection and quantitation of infectious pancreatic necrosis virus by real-time reverse transcriptase-polymerase chain reaction using lethal and non-lethal tissue sampling;Bowers;Journal of Virological Methods,2008
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