Affiliation:
1. Beijing Key Laboratory of Development and Quality Control of Ornamental Crops, Department of Ornamental Horticulture China Agricultural University Beijing China
2. Institute of Grassland, Flowers, and Ecology Beijing Academy of Agriculture and Forestry Sciences Beijing China
Abstract
AbstractGladiolus hybridus is one of the most popular flowers worldwide. However, its corm dormancy characteristic largely limits its off‐season production. Long‐term cold treatment (LT), which increases sugar content and reduces abscisic acid (ABA), is an efficient approach to accelerate corm dormancy release (CDR). Here, we identified a GhbZIP30‐GhCCCH17 module that mediates the antagonism between sugars and ABA during CDR. We showed that sugars promoted CDR by reducing ABA levels in Gladiolus. Our data demonstrated that GhbZIP30 transcription factor directly binds the GhCCCH17 zinc finger promoter and activates its transcription, confirmed by yeast one‐hybrid, dual‐luciferase (Dual‐LUC), chromatin immunoprecipitation‐quantitative PCR (ChIP‐qPCR) and electrophoretic mobility shift assay (EMSA). GhCCCH17 is a transcriptional activator, and its nuclear localisation is altered by surcose and cytokinin treatments. Both GhbZIP30 and GhCCCH17 positively respond to LT, sugars, and cytokinin treatments. Silencing GhbZIP30 or GhCCCH17 resulted in delayed CDR by regulating ABA metabolic genes, while their overexpression promoted CDR. Taken together, we propose that the GhbZIP30‐GhCCCH17 module is involved in cold‐ and glucose‐induced CDR by regulating ABA metabolic genes.
Funder
National Natural Science Foundation of China
Cited by
3 articles.
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