Multiplexed phosphospecific flow cytometry enables large-scale signaling profiling and drug screening in blood platelets

Author:

Spurgeon B. E. J.1,Aburima A.1,Oberprieler N. G.2,Taskén K.2345,Naseem K. M.1

Affiliation:

1. Centre for Cardiovascular and Metabolic Research; Hull York Medical School; University of Hull; Hull UK

2. Center for Molecular Medicine Norway; Nordic EMBL Partnership; University of Oslo and Oslo University Hospital; Oslo Norway

3. Biotechnology Center; University of Oslo; Oslo Norway

4. K. G. Jebsen Inflammation Research Center; University of Oslo; Oslo Norway

5. Department of Infectious Diseases; Oslo University Hospital; Oslo Norway

Funder

Hull York Medical School

Publisher

Wiley

Subject

Hematology

Reference23 articles.

1. Arterial thrombosis - insidious, unpredictable and deadly;Jackson;Nat Med,2011

2. Platelet protein interactions: map, signaling components, and phosphorylation groundstate;Dittrich;Arterioscler Thromb Vasc Biol,2008

3. Fluorescent cell barcoding in flow cytometry allows high-throughput drug screening and signaling profiling;Krutzik;J Immunol,2006

4. High-content single-cell drug screening with phosphospecific flow cytometry;Krutzik;Nat Chem Biol,2008

5. cAMP- and cGMP-dependent protein kinase phosphorylation sites of the focal adhesion vasodilator-stimulated phosphoprotein (VASP) in vitro and in intact human platelets;Butt;J Biol Chem,1994

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