Affiliation:
1. Bloom Classic Co., Ltd. Ehime Japan
2. Department of Dermatology, Faculty of Medicine Osaka Medical and Pharmaceutical University Osaka Japan
Abstract
AbstractBackgroundWhen the skin is damaged and its barrier function is disrupted, the proliferation and migration of epidermal keratinocytes are vital for repairing the damaged area. The Schumann resonance at 7.8 Hz has been reported to protect rat cardiomyocytes against oxidative stress and inhibit the proliferation of B16 mouse melanoma cells. However, its effect on the skin is unknown.AimsIn this study, we applied 7.8‐Hz electromagnetic waves to normal human epidermal keratinocytes (NHEKs) and investigated its effects on cell proliferation and migration, β‐defensin (DEFB1) and sirtuin 1 (SIRT1) expression.MethodsWe performed cell proliferation assay, cell migrationassay and gene expression analysis of DEFB1 and SIRT1.ResultsWe found that the application of 7.8‐Hz electromagnetic waves caused a 2.8‐fold increase in NHEK proliferation, enhanced cell migration, and increased the expression of DEFB1 and SIRT1 by 2.4‐fold and 4.9‐fold, respectively.ConclusionsThese results suggest that the application of 7.8‐Hz electromagnetic waves may contribute to improving the skin barrier function and skin ulcer.