Affiliation:
1. Department of Food Sciences, Faculty of Science and Technology Universiti Kebangsaan Malaysia 43600 UKM Bangi Selangor Malaysia
2. Centre of Excellence, Innovation Centre for Confectionery Technology (MANIS), Faculty of Science and Technology Universiti Kebangsaan Malaysia 43600 UKM Bangi Selangor Malaysia
3. Herbal Medicine Research Centre Institute for Medical Research, National Institutes of Health, Ministry of Health Malaysia Shah Alam 40170 Selangor Malaysia
4. Institute of System Biology Universiti Kebangsaan Malaysia 43600 UKM Bangi Selangor Malaysia
Abstract
SummaryAlpinia mutica Roxb. possesses diverse phytochemicals with potential as treatment modalities in managing diabetes and obesity. Employing a nuclear magnetic resonance (NMR)‐based metabolomics, we conducted discriminative analysis to investigate the metabolite profiles across different extraction solvents (ethanol:water −100:0, 80:20, 60:40, 40:60, 20:80 and 0:100) and correlated these profiles with bioactivities. Metabolites were identified using NMR supplemented with liquid chromatography tandem mass spectrometry (LC–MS/MS) analysis. The 40:60 extract showed high phenolic content, strong ferric ion reduction capability and low IC50 value in scavenging free radicals, pancreatic lipase and α‐glucosidase inhibitory assays. Partial Least Square (PLS) biplot indicated that isorhamnetin, catechin, zerumin B, curcumanggoside, curcuzederone, demethoxycurcumin, ascorbic acid, apigenin, and phenylalanine in the 40:60 extract exhibited good correlations with the measured bioactivities. Additionally, LC–MS/MS tentatively identified proanthocyanidins and multiple flavonoid glucuronides in this extract. This study provides preliminary insight into its chemistry and highlight the potential of A. mutica extract as natural antioxidant and antiobesity agent.
Funder
Ministry of Higher Education, Malaysia