Purification, characterisation and immobilisation of a protease‐resistant α‐galactosidase from Monascus purpureus and its application in removal of raffinose family oligosaccharides

Author:

Zhu Chong1,Katrolia Priti1ORCID,Kopparapu Narasimha Kumar1

Affiliation:

1. College of Food Science Southwest University No. 2 Tiansheng Road, Beibei District Chongqing 400715 China

Abstract

SummaryIn this study, an α‐galactosidase (MPG) was produced by submerged fermentation from Monascus purpureus, a food‐grade fungus used for making red yeast rice. MPG was purified from intracellular extract which showed a subunit molecular mass of 95 kDa. MPG was optimally active at pH 4.0 and 50 °C and was stable between pH 3.5–8.0 and at temperatures up to 50 °C. The enzyme retained its activity in the presence of most of the metal ions except Cu2+ and Fe2+. MPG exhibited remarkable resistance to the proteases, pepsin, trypsin and proteinase K as well as to the sugars, galactose and sucrose. Moreover, it retained complete activity under simulated gastric conditions. The enzyme displayed specificity for the substrates, melibiose, raffinose and stachyose and was able to hydrolyze these oligosaccharides. It was effective in the removal of non‐digestible raffinose family oligosaccharides (RFOs) from soybeans, chickpeas and kidney beans. MPG was immobilised onto chitosan and the immobilised enzyme (iMPG) displayed higher optimal temperature (60 °C), better thermostability and substrate specificity as compared to MPG. iMPG was also capable of hydrolyzing RFOs in chickpeas and could be efficiently reused six times. In view of their superior properties, MPG and iMPG may have great prospects in the food and feed industries for the removal of RFOs from soy and other legumes.

Publisher

Wiley

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