Affiliation:
1. Laboratorio de Neuro‐inmuno‐endocrinología testicular, Instituto de Biología y Medicina Experimental (IBYME), Fundación IBYME, CONICET Ciudad de Buenos Aires Argentina
2. Cátedra 1, Bioquímica Humana, Facultad de Medicina Universidad de Buenos Aires Ciudad de Buenos Aires Argentina
3. Fertilis Medicina Reproductiva San Isidro Buenos Aires Argentina
4. PREGNA Medicina Reproductiva Ciudad de Buenos Aires Argentina
5. Cátedra de Química, Ciclo Básico Común Universidad de Buenos Aires Ciudad de Buenos Aires Argentina
Abstract
AbstractBackgroundThe implications of SARS‐CoV‐2 infection on male fertility remain largely unknown. Besides their well‐known pro‐ and anti‐inflammatory actions, prostaglandins and polyamines are present in semen, where they play key roles in sperm quality.ObjectivesTo analyze semen parameters, oxidative profile and the seminal fluid prostaglandin and polyamine systems in samples collected from individuals without coronavirus disease 2019 diagnosis and men who recovered from coronavirus disease 2019.Materials and methodsThis study compared semen collected from men without positive coronavirus disease 2019 diagnosis with samples obtained from individuals 1–6 months and 7–30 months post SARS‐CoV‐2 infection. Semen parameters, thiobarbituric acid reactive substances, cyclooxygenase 2 expression by fluorescence immunocytochemistry and immunoblotting, prostaglandin levels by enzyme immunoassay, ornithine decarboxylase activity by a radioactive assay, and polyamine and acetylated polyamine levels by thin‐layer chromatography were assessed.ResultsIn both groups of semen samples from coronavirus disease 2019 recovered men, sperm vitality, total and progressive sperm motility, and putrescine levels were significantly decreased when compared with samples from the uninfected group. In contrast, lipid peroxidation, leukocyte‐associated cyclooxygenase 2 expression, and prostaglandin D2 levels were higher in semen from coronavirus disease 2019 recovered men than in samples from uninfected individuals. While sperm concentration and morphology, ornithine decarboxylase activity, and N‐acetylputrescine levels were statistically diminished in semen obtained up to 6 months after coronavirus disease 2019 recovery, these parameters remained unchanged when samples were collected 7–30 months after coronavirus disease 2019 recovery. Coronavirus disease 2019 vaccination did not show negative effects on any of the parameters evaluated.Discussion and conclusionOur work provides insights into the detrimental impact of coronavirus disease 2019 on several sperm parameters, in some cases, even more than a year after SARS‐CoV‐2 infection, which would be accompanied by alterations in the seminal fluid prostaglandin and polyamine profiles. Therefore, future treatments targeting the prostaglandin and polyamine pathways in coronavirus disease 2019 recovered men could lead to a successful reinstatement of semen parameters.
Funder
Consejo Nacional de Investigaciones Científicas y Técnicas
Agencia Nacional de Promoción Científica y Tecnológica
Fundación Alberto J. Roemmers
Fundación René Barón
Subject
Urology,Endocrinology,Reproductive Medicine,Endocrinology, Diabetes and Metabolism
Cited by
1 articles.
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1. Age-Related COVID-19 Influence on Male Fertility;International Journal of Molecular Sciences;2023-10-30