Optimized metabarcoding with Pacific biosciences enables semi‐quantitative analysis of fungal communities

Author:

Castaño Carles1ORCID,Berlin Anna1ORCID,Brandström Durling Mikael1ORCID,Ihrmark Katharina1,Lindahl Björn D.2ORCID,Stenlid Jan1ORCID,Clemmensen Karina E.1ORCID,Olson Åke1ORCID

Affiliation:

1. Department of Forest Mycology and Plant Pathology Swedish University of Agricultural Sciences UppsalaSE‐75007Sweden

2. Department of Soil and Environment Swedish University of Agricultural Sciences UppsalaSE‐75007Sweden

Funder

Svenska Forskningsrådet Formas

Vetenskapsrådet

Publisher

Wiley

Subject

Plant Science,Physiology

Reference47 articles.

1. ITS all right mama: investigating the formation of chimeric sequences in the ITS2 region by DNA metabarcoding analyses of fungal mock communities of different complexities;Aas AB;Molecular Ecology Resources,2016

2. Quantifying microbial communities with 454 pyrosequencing: does read abundance count?

3. Great differences in performance and outcome of high-throughput sequencing data analysis platforms for fungal metabarcoding

4. A fungal mock community control for amplicon sequencing experiments

5. Estimation of fungal biomass in forest litter and soil

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