MdWER interacts with MdERF109 and MdJAZ2 to mediate methyl jasmonate‐ and light‐induced anthocyanin biosynthesis in apple fruit

Author:

Zhang Xi12,Yu Lujia12,Zhang Mengjiao12,Wu Ting3ORCID,Song Tingting12,Yao Yuncong12,Zhang Jie12,Tian Ji12ORCID

Affiliation:

1. Beijing Advanced Innovation Center for Tree Breeding by Molecular Design Beijing University of Agriculture Beijing China

2. Plant Science and Technology College, Beijing University of Agriculture Beijing China

3. College of Horticulture China Agricultural University Beijing China

Abstract

SUMMARYAnthocyanin generation in apples (Malus domestica) and the pigmentation that results from it may be caused by irradiation and through administration of methyl jasmonate (MeJA). However, their regulatory interrelationships associated with fruit coloration are not well defined. To determine whether MdERF109, a transcription factor (TF) involved in light‐mediated coloration and anthocyanin biosynthesis, has synergistic effects with other proteins, we performed a yeast two‐hybrid assessment and identified another TF, MdWER. MdWER was induced by MeJA treatment, and although overexpression of MdWER alone did not promote anthocyanin accumulation co‐overexpression with MdERF109 resulted in significantly increase in anthocyanin biosynthesis. MdWER may form a protein complex with MdERF109 to promote anthocyanin accumulation by enhancing combinations between the proteins and their corresponding genes. In addition, MdWER, as a MeJA responsive protein, interacts with the anthocyanin repressor MdJAZ2. Transient co‐expression in apple fruit and protein interaction assays allowed us to conclude that MdERF109 and MdJAZ2 interact with MdWER and take part in the production of anthocyanins upon MeJA treatment and irradiation. Our findings validate a role for the MdERF109‐MdWER‐MdJAZ2 module in anthocyanin biosynthesis and uncover a novel mechanism for how light and MeJA signals are coordinated anthocyanin biosynthesis in apple fruit.

Funder

Beijing Nova Program

Beijing University of Agriculture

National Natural Science Foundation of China

Publisher

Wiley

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