Effect of non‐selenium glutathione peroxidase activity of peroxiredoxin 6 on beef tenderness: underlying mechanisms

Author:

Huang Linlin123ORCID,Wang Xinyi12ORCID,Holman Benjamin W. B.4ORCID,Dong Pengcheng12ORCID,Zhang Yimin12ORCID,Zhu Lixian12ORCID,Luo Xin12ORCID,Mao Yanwei12ORCID

Affiliation:

1. College of Food Science and Engineering Shandong Agricultural University Tai'an Shandong 271018 China

2. National R&D Center for Beef Processing Technology Tai'an Shandong 271018 China

3. Jining Institute for Food and Drug Control Jining Shandong 272073 China

4. NSW Department of Primary Industries Wagga Wagga Agricultural Institute Wagga Wagga New South Wales 2650 Australia

Abstract

SummaryThis study investigated the effect of non‐selenium glutathione peroxidase (NSGPx) activity of Peroxiredoxin 6 (Prdx6) on beef tenderness. Longissimus thoracis et lumborum (LTL) muscle from six cattle were obtained at 30 min post‐mortem. Samples from each muscle were randomly assigned to each of four treatment groups: being incubated in H2O2 (simulation of oxidative stress), mercaptosuccinic acid (MA; inhibiting NSGPx activity), N‐acetyl‐L‐cysteine (NAC; inhibiting oxidative stress and increasing NSGPx activity), and saline (control), respectively. Results indicated that cells increased the expression of Prdx6 and NSGPx activity to resist oxidative stress. The inhibition of NSGPx activity group (MA) significantly decreased the Warner‐Bratzler shear force and increased the degradation of desmin. Moreover, both the H2O2 and the MA treatment significantly increased the Ca2+ concentration in the sarcoplasm, cell apoptotic rate, and caspase 9 and 3 activity. These results demonstrated that inhibiting NSGPx activity of Prdx6 could accelerate beef tenderization by promoting mitochondrial apoptosis.

Publisher

Wiley

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